CPC Class C12Q

Methods and compositions are disclosed relating to the localization of nucleic acids to arrays such as silane-free arrays, and of sequencing the nucleic acids localized thereby.

The present invention provides methods, compositions, and systems for distributing single polymerase molecules into array regions. In particular, the methods, compositions, and systems of the present invention result in a distribution of single polymerase molecules into array regions at a percentage that is larger than the percentage expected to be occupied under a Poisson distribution.

A method for producing a microbiologic agent is described. The method includes a step of adding biomass containing a target compound-degrading microorganism and an inorganic microparticle carrier to a medium containing a target compound. The microorganisms included in the biomass are carried on the inorganic microparticles, and the medium is cultured while monitoring an abundance ratio of the target compound-degrading microorganism in microorganisms carried on the inorganic microparticle carrier. After the abundance ratio of the target compound-degrading microorganism reaches a predetermined value, the inorganic microparticle carrier carrying the target compound-degrading microorganism is collected to obtain the microbiologic agent.

The present invention relates generally to glutaminase inhibitors of Formula I, Formula II, or Formula III, as well as pharmaceutical compounds containing them and methods of their use.