CPC Class C12Q

The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

Methods are provided for targeting cells for depletion, including, without limitation, cancer cells, in a regimen comprising contacting the targeted cells with a combination of agents, including (i) an agent that blockades CD47 activity; and (ii) an antibody that specifically binds to EGFR. In some embodiments the cancer cells have a mutated form of one or more of KRAS, NRAS and BRAF. The level of depletion of the targeted cell is enhanced relative to a regimen in which a single agent is used; and the effect may be synergistic relative to a regimen in which a single agent is used.

The present invention relates to methods for isolating nucleic acids present in a sample, in particular cell-free DNA (cfDNA) from a blood sample and polymers, substrates and kits for the method. Polymers with characteristics suitable to bind such nucleic acids are provided.

The present invention provides sequence specific restriction enzymes for site-specific cleavage of RNA, as well as methods of their use.

The present invention relates to a DNA-peptide complex with a high-density functional group, a DNA-peptide-nanomaterial complex and a method for production of the same, a DNA-metal nanowire, and a method for production of the same. The DNA-peptide complex with a high-density functional group may include a DNA molecule; and a peptide containing an amino acid sequence capable of binding to the DNA molecule, wherein the peptide contains at least one functional group at a terminal thereof, wherein the peptide binds to the DNA molecule via at least one of electrostatic interaction, intercalation, and groove binding.