CPC Class C12Q

The present disclosure relates to an oxidation-reduction polymer which can be used in an electrochemical sensor, and particularly, in a polymer backbone of an electron transfer medium of the electrochemical sensor. More specifically, the present disclosure relates to: an oxidation-reduction polymer which can be used in a poly (allyl glycidyl ether)-based electrochemical sensor including a repeating unit derived from allyl glycidyl ether; and an electron transfer medium and an electrochemical sensor including same, wherein the oxidation-reduction polymer is advantageous in confirming the completion of reaction during manufacture, has high immobilization efficiency of the transition metal complex, has low possibility of having problems of toxicity and side effects, and can add various functions.

The present invention provides methods and kits for efficiently generating edited DNA, ranging from DNA molecules with simple point mutations to complex DNA libraries. The methods and kits use a single-stranded deoxyuridine (dU)-containing template to align a series of DNA oligomers that contain mutations relative to the template strand, giving rise to the sequence variation in the product. The methods and kits may be used to produce DNA libraries or to generate multiple, widely spaced mutations in a target sequence.

A method of screening is provided including providing a combination of a plurality of proliferating cell types wherein each proliferating cell type has a unique associated barcode within its genome that is different from other proliferating cell types of the plurality and wherein each proliferating cell type includes an exogenous gene that when expressed produces an associated phenotype to the proliferating cell type which alters proliferation of the cell type, introducing a perturbation to one or more of the plurality of proliferating cell types, inducing expression of one or more of the exogenous genes, determining the relative number of unique associated barcodes after a period of proliferation, and comparing the relative number of unique associated barcodes to a control relative number of unique associated barcodes to indicate the effect of the perturbation on the one or more of the plurality of proliferating cell types.

The present invention concerns bioactive renal cell populations, renal cell constructs, and methods of making and using the same.

The present invention relates to compositions and methods for detecting motor neuron-specific miRNAs in a population of cells or subject. More particularly, the invention relates to detecting motor neuron-specific miRNAs to detect and treat motor neuron diseases associated with dysregulation of motor neuron-specific miRNAs, such as Amyotrophic Lateral Sclerosis (ALS).